Tuesday, July 23, 2013

Lab 7: Gel-electrophoresis and Genetic Architecture

Materials

  • agar gel
  • human blood samples (normal, carrier, sickle trait)
  • gel-electrophoresis apparatus
  • slide of normal red blood cells and sickle cells
  • computer for transcription and translation simulation
  • beads for constructing proteins based on the results of the simulation

Exercises

  • Conduct gel-electrophoresis on 3 samples of human blood
  • Transcription and translation simulation

Materials

  • Major body parts for building a hypothetical Rebop organism
  • Antennae, mouth, eyes, legs, etc. with a genetic basis
  • Chromosomes with genes
  • Computer laboratory exercise on karyotyping

Exercises

  • Build a hypothetical organism based on randomly selected genetic information
  • Try to determine the phenotype of the parents of the hypothetical organism
  • Try to determine the genotype and phenotype of the offspring of a mating with another organism from within your lab
  • Conduct a karyotyping exercise matching chromosomes from a computer simulation

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